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Granulocyte Marker Antibody (SPM250) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48057

Novus Biologicals, part of Bio-Techne
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NBP2-48057

Key Product Details

Species Reactivity

Human, Rhesus Macaque

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM250

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes.

Reactivity Notes

Macaque Monkey.

Localization

Cytoplasmic

Specificity

Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. monoclonal antibody BM-2 reacts with early precursor and mature forms of human myeloid cells. This monoclonal antibody is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.

Marker

Macrophage / Granulocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Granulocyte Marker Antibody (SPM250) - IHC-Prediluted

Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - IHC-Prediluted [NBP2-48057]

Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - IHC-Prediluted [NBP2-48057]

Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - IHC-Prediluted [NBP2-48057] - Formalin-fixed, paraffin-embedded tonsil stained with Granulocyte MAb (SPM250). Note specific cytoplasmic staining.

Applications for Granulocyte Marker Antibody (SPM250) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Granulocyte Marker

Alternate Names

Granulocytes;PML;PMN

Additional Granulocyte Marker Products

Product Documents for Granulocyte Marker Antibody (SPM250) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Granulocyte Marker Antibody (SPM250) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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