Skip to main content

Golgi Complex Antibody (SPM581) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34813

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM581

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

SU-DHL-1 large cell lymphoma cells.

Localization

Golgi complex in cytoplasm

Specificity

This monoclonal antibody recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.

Marker

Marker for Human Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-32853).

Antibody with azide - store at 2 to 8C.

Scientific Data Images for Golgi Complex Antibody (SPM581) - Azide and BSA Free

Immunohistochemistry-Paraffin: Golgi Complex Antibody (SPM581) - Azide and BSA Free [NBP2-34813]

Immunohistochemistry-Paraffin: Golgi Complex Antibody (SPM581) - Azide and BSA Free [NBP2-34813]

Immunohistochemistry-Paraffin: Golgi Complex Antibody (SPM581) - Azide and BSA Free [NBP2-34813] - Formalin-fixed, paraffin-embedded human Gallbladder stained with Golgi Monoclonal Antibody (SPM581).

Applications for Golgi Complex Antibody (SPM581) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml
Application Notes
Immunocytochemistry (Acetone or paraformaldehyde fixed): 1-2ug/ml for 30 min.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Golgi Complex

Alternate Names

AI593353;AW537898;CFR;CFR1;CFR-1;ESL1;ESL-1;GLG1;MG160;MG-160;Selel

Additional Golgi Complex Products

Product Documents for Golgi Complex Antibody (SPM581) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Golgi Complex Antibody (SPM581) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...