Skip to main content

Key Product Details

Species Reactivity

Human, Mouse (Negative), Rat (Negative)

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # GLG1/2829R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human Golgi Complex protein (Uniprot: Q92896)

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Golgi complex in cytoplasm

Specificity

This monoclonal antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

Marker

Marker for Human Cells

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP3-07278).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Golgi Complex Antibody (GLG1/2829R) - Azide and BSA Free

Immunohistochemistry-Paraffin: Golgi Complex Antibody (GLG1/2829R) - Azide and BSA Free [NBP3-08396]

Immunohistochemistry-Paraffin: Golgi Complex Antibody (GLG1/2829R) - Azide and BSA Free [NBP3-08396]

Immunohistochemistry-Paraffin: Golgi Complex Antibody (GLG1/2829R) - Azide and BSA Free [NBP3-08396] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Golgi Complex Rabbit Recombinant Monoclonal Antibody (GLG1/2829R).

Applications for Golgi Complex Antibody (GLG1/2829R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Golgi Complex

Alternate Names

AI593353;AW537898;CFR;CFR1;CFR-1;ESL1;ESL-1;GLG1;MG160;MG-160;Selel

Additional Golgi Complex Products

Product Documents for Golgi Complex Antibody (GLG1/2829R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Golgi Complex Antibody (GLG1/2829R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...