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GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43552

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-43552

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # KM966

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

This chimeric antibody was generated by cloning the variable heavy and light chain sequences of murine parent antibody KM696 (IgM) and expressing it as a mouse/human IgG1. The parent antibody was raised against human GM2.

Specificity

This antibody recognizes and binds ganglioside GM2.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Scientific Data Images for GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free

GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free

Immunocytochemistry /Immunofluorescence: GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free [NBP3-43552] -

Immunocytochemistry /Immunofluorescence: GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free [NBP3-43552] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with NBP3-43552 (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43552, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (NBP2-52638) followed by staining with Alexa Fluor 488 secondary antibody.

Applications for GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GM2 Ganglioside

Alternate Names

GM2 (ganglioside), Tay-Sachs ganglioside

Additional GM2 Ganglioside Products

Product Documents for GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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