Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Analysis in human placenta and skeletal muscle tissues using NBP3-18542 antibody. Corresponding GPC3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human liver cancer (hepatocellular carcinoma) shows moderate membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human ovarian cancer (papillary carcinoma) shows moderate membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human testis cancer (seminoma) shows moderate membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human ovarian cancer (yolk sack tumor) shows moderate membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human kidney shows moderate membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

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Applications for Glypican 3 Antibody (CL12711) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 -1:2500

Application Notes

IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glypican 3

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Alternate Names

GPC3

Gene Symbol

GPC3

Additional Glypican 3 Products

Product Documents for Glypican 3 Antibody (CL12711) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Glypican 3 Antibody (CL12711) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Glypican 3 Antibody (CL12711) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18542

Key Product Details

Species Reactivity

Validated:

Predicted:

Applications

Label

Antibody Source

Format

Concentration

View all Glypican 3 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for Glypican 3 Antibody (CL12711) - BSA Free

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Applications for Glypican 3 Antibody (CL12711) - BSA Free

Immunohistochemistry

Immunohistochemistry-Paraffin

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Glypican 3

Alternate Names

Gene Symbol

Additional Glypican 3 Products

Product Documents for Glypican 3 Antibody (CL12711) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Glypican 3 Antibody (CL12711) - BSA Free