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GLP1 Antibody (10) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05180B

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mammal

Applications

Western Blot, ELISA

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 10

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic GLP-1(7-17)

Epitope

Free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide.

Reactivity Notes

The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Specificity

Binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for GLP1 Antibody (10) [Biotin]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well. It binds free exendin-4 in solution and can function as a capture antibody for exendin-4. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM Phosphate buffer (pH 7.4) and 0.14 M NaCl

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: GLP-1

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Long Name

Glucagon-like Peptide 1

Alternate Names

GLP1

Gene Symbol

GCG

Additional GLP-1 Products

Product Documents for GLP1 Antibody (10) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GLP1 Antibody (10) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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