GIT1 Antibody (S39B-8) - BSA Free

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423]

Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GIT1 Antibody. (D) Composite.

Western Blot: GIT1 Antibody (S39B-8) - BSA Free [NBP2-22423] -

The role of GIT1 in autophagy of osteoclasts and HEK293T cells.a Amino-acid starvation in Earle’s balanced salt solution (EBSS) for different time durations (0, 0.5, 1.0, and 2.0 h) progressively increased the expression of GIT1 and LC3-II in osteoclasts. b The expression of GIT1 and LC3-II were both gradually upregulated under amino-acid starvation conditions in EBSS for different time durations (0, 1.0, and 2.0 h) in HEK293T cells. c The effectiveness of siRNA1 in knocking down GIT1 expression in osteoclasts under basal and starvation conditions. si-NC was used as the control. d The efficacy of GIT1-HA in overexpression of GIT1 in HEK293T cells under non-starvation and starvation conditions. e, f The effect of GIT1 knockdown in lowering the LC3-II level under non-starvation or starvation conditions (1 h) with or without bafilomycin A1 (Baf, 10 nM) in osteoclasts. Bafilomycin A1 was used to inhibit LC3-II degradation. Representative immunoblot images (e) and data summary (f) are shown (*p < 0.05, **p < 0.01, ns indicates no significance, Kruskal–Wallis test). g, h The effect of GIT1 overexpression in increasing the LC3-II level under basal or starvation conditions with or without bafilomycin A1 (Baf, 10 nM) in HEK293T cells. Representative images (g) and data summary (h) are shown (**p < 0.01, Kruskal–Wallis test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30546041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GIT1 Antibody (S39B-8) - BSA Free [NBP2-22423] -

The role of GIT1 in autophagy of osteoclasts and HEK293T cells.a Amino-acid starvation in Earle’s balanced salt solution (EBSS) for different time durations (0, 0.5, 1.0, and 2.0 h) progressively increased the expression of GIT1 and LC3-II in osteoclasts. b The expression of GIT1 and LC3-II were both gradually upregulated under amino-acid starvation conditions in EBSS for different time durations (0, 1.0, and 2.0 h) in HEK293T cells. c The effectiveness of siRNA1 in knocking down GIT1 expression in osteoclasts under basal and starvation conditions. si-NC was used as the control. d The efficacy of GIT1-HA in overexpression of GIT1 in HEK293T cells under non-starvation and starvation conditions. e, f The effect of GIT1 knockdown in lowering the LC3-II level under non-starvation or starvation conditions (1 h) with or without bafilomycin A1 (Baf, 10 nM) in osteoclasts. Bafilomycin A1 was used to inhibit LC3-II degradation. Representative immunoblot images (e) and data summary (f) are shown (*p < 0.05, **p < 0.01, ns indicates no significance, Kruskal–Wallis test). g, h The effect of GIT1 overexpression in increasing the LC3-II level under basal or starvation conditions with or without bafilomycin A1 (Baf, 10 nM) in HEK293T cells. Representative images (g) and data summary (h) are shown (**p < 0.01, Kruskal–Wallis test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30546041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GIT1 Antibody (S39B-8) - BSA Free [NBP2-22423] -

The role of GIT1 in autophagy of osteoclasts and HEK293T cells.a Amino-acid starvation in Earle’s balanced salt solution (EBSS) for different time durations (0, 0.5, 1.0, and 2.0 h) progressively increased the expression of GIT1 and LC3-II in osteoclasts. b The expression of GIT1 and LC3-II were both gradually upregulated under amino-acid starvation conditions in EBSS for different time durations (0, 1.0, and 2.0 h) in HEK293T cells. c The effectiveness of siRNA1 in knocking down GIT1 expression in osteoclasts under basal and starvation conditions. si-NC was used as the control. d The efficacy of GIT1-HA in overexpression of GIT1 in HEK293T cells under non-starvation and starvation conditions. e, f The effect of GIT1 knockdown in lowering the LC3-II level under non-starvation or starvation conditions (1 h) with or without bafilomycin A1 (Baf, 10 nM) in osteoclasts. Bafilomycin A1 was used to inhibit LC3-II degradation. Representative immunoblot images (e) and data summary (f) are shown (*p < 0.05, **p < 0.01, ns indicates no significance, Kruskal–Wallis test). g, h The effect of GIT1 overexpression in increasing the LC3-II level under basal or starvation conditions with or without bafilomycin A1 (Baf, 10 nM) in HEK293T cells. Representative images (g) and data summary (h) are shown (**p < 0.01, Kruskal–Wallis test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30546041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GIT1 Antibody (S39B-8) - BSA Free [NBP2-22423] -

The role of GIT1 in autophagy of osteoclasts and HEK293T cells.a Amino-acid starvation in Earle’s balanced salt solution (EBSS) for different time durations (0, 0.5, 1.0, and 2.0 h) progressively increased the expression of GIT1 and LC3-II in osteoclasts. b The expression of GIT1 and LC3-II were both gradually upregulated under amino-acid starvation conditions in EBSS for different time durations (0, 1.0, and 2.0 h) in HEK293T cells. c The effectiveness of siRNA1 in knocking down GIT1 expression in osteoclasts under basal and starvation conditions. si-NC was used as the control. d The efficacy of GIT1-HA in overexpression of GIT1 in HEK293T cells under non-starvation and starvation conditions. e, f The effect of GIT1 knockdown in lowering the LC3-II level under non-starvation or starvation conditions (1 h) with or without bafilomycin A1 (Baf, 10 nM) in osteoclasts. Bafilomycin A1 was used to inhibit LC3-II degradation. Representative immunoblot images (e) and data summary (f) are shown (*p < 0.05, **p < 0.01, ns indicates no significance, Kruskal–Wallis test). g, h The effect of GIT1 overexpression in increasing the LC3-II level under basal or starvation conditions with or without bafilomycin A1 (Baf, 10 nM) in HEK293T cells. Representative images (g) and data summary (h) are shown (**p < 0.01, Kruskal–Wallis test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30546041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GIT1 Antibody (S39B-8) - BSA Free [NBP2-22423] -

GIT1 physically interacts with Beclin1 and contributes to the disruption of Beclin1-Bcl2 binding in HEK293T cells.a–d Co-IP assays between GIT1 and Beclin1 under basal and starvation conditions. The pulling antibodies (anti-GIT1 antibody in a and anti-Beclin1 antibody in c) and the blotting antibodies are indicated. The cell lysates are displayed as input, and IgG is used as an internal control (values are means +/- SD, **p < 0.01, two-tailed Student's t-tests). e, f Co-IP assays between Beclin1 and Bcl2 with or without GIT1 under basal and starvation conditions. The pulling antibodies (anti-Beclin1 antibody) and the blotting antibodies are indicated. The cell lysates are displayed as input, and IgG is used as an internal control (values are means +/- SD, **p < 0.01, two-tailed Student's t-tests) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30546041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GIT1 Antibody (S39B-8) - BSA Free [NBP2-22423] -

GIT1 physically interacts with Beclin1 and contributes to the disruption of Beclin1-Bcl2 binding in HEK293T cells.a–d Co-IP assays between GIT1 and Beclin1 under basal and starvation conditions. The pulling antibodies (anti-GIT1 antibody in a and anti-Beclin1 antibody in c) and the blotting antibodies are indicated. The cell lysates are displayed as input, and IgG is used as an internal control (values are means +/- SD, **p < 0.01, two-tailed Student's t-tests). e, f Co-IP assays between Beclin1 and Bcl2 with or without GIT1 under basal and starvation conditions. The pulling antibodies (anti-Beclin1 antibody) and the blotting antibodies are indicated. The cell lysates are displayed as input, and IgG is used as an internal control (values are means +/- SD, **p < 0.01, two-tailed Student's t-tests) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30546041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody (S39B-8) - BSA Free [NBP2-22423] -

Overexpression of GIT1 promoted autophagic flux in HEK293T cells in vitro.a, b The control and GIT1-overexpressing HEK293T cells were transfected with mRFP–GFP–LC3. Effect of GIT1 overexpression in promoting LC3 puncta formation under non-starvation and starvation conditions. Shown are LC3 fluorescent signals from representative single cells. Representative images (a) and data summary (b) are shown (**p < 0.01, Kruskal–Wallis test). Scale bar = 20 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30546041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.