GCR2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33278
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit GCR2 Antibody - BSA Free (NBP2-33278) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for GCR2 Antibody - BSA Free
![Western Blot: GCR2 Antibody [NBP2-33278]](https://resources.bio-techne.com/images/products/GCR2-Antibody-Western-Blot-NBP2-33278-img0007.jpg "Western Blot: GCR2 Antibody [NBP2-33278]")
Western Blot: GCR2 Antibody [NBP2-33278]
Western Blot: GCR2 Antibody [NBP2-33278] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp![Immunocytochemistry/ Immunofluorescence: GCR2 Antibody [NBP2-33278]](https://resources.bio-techne.com/images/products/GCR2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33278-img0008.jpg "Immunocytochemistry/ Immunofluorescence: GCR2 Antibody [NBP2-33278]")
Immunocytochemistry/ Immunofluorescence: GCR2 Antibody [NBP2-33278]
Immunocytochemistry/Immunofluorescence: GCR2 Antibody [NBP2-33278] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.![GCR2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: GCR2 Antibody - BSA Free [NBP2-33278]](https://resources.bio-techne.com/images/products/nbp2-33278_rabbit-polyclonal-gcr2-antibody-25620259114043.jpg "Chromatin Immunoprecipitation-exo-Seq: GCR2 Antibody - BSA Free [NBP2-33278]")
Chromatin Immunoprecipitation-exo-Seq: GCR2 Antibody - BSA Free [NBP2-33278]
ChIP-Exo-Seq composite graph for Anti-ECD (NBP2-33278) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for GCR2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GCR2
Alternate Names
ecdysoneless homolog (Drosophila), GCR2, HSGT1, Protein ecdysoneless homolog, protein SGT1, SGT1, Suppressor of GCR2, suppressor of S. cerevisiae gcr2, X
Gene Symbol
ECD
UniProt
Additional GCR2 Products
Product Documents for GCR2 Antibody - BSA Free
Product Specific Notices for GCR2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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