GAPDH Antibody (CL3265)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52086

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-52086-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL3265

Concentration

1 mg/ml

View all GAPDH Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P04406, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GAPDH Antibody (CL3265)

GAPDH Antibody (CL3265) Immunohistochemistry: GAPDH Antibody (CL3265) [NBP3-52086]

Immunohistochemistry: GAPDH Antibody (CL3265) [NBP3-52086]

Staining of human kidney shows strong cytoplasmic and nuclear positivity in renal tubules and glomeruli.
GAPDH Antibody (CL3265) Immunohistochemistry: GAPDH Antibody (CL3265) [NBP3-52086]

Immunohistochemistry: GAPDH Antibody (CL3265) [NBP3-52086]

Staining of human liver shows strong cytoplasmic and nuclear immunoreactivity in hepatocytes.
GAPDH Antibody (CL3265) Western Blot: GAPDH Antibody (CL3265) [NBP3-52086]

Western Blot: GAPDH Antibody (CL3265) [NBP3-52086]

Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.
View 4 more images

Applications for GAPDH Antibody (CL3265)

Application
Recommended Usage

Immunohistochemistry

1:2500-1:5000

Immunohistochemistry-Paraffin

1:2500-1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAPDH

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References

1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase

Alternate Names

G3PDH

Gene Symbol

GAPDH

Additional GAPDH Products

Product Documents for GAPDH Antibody (CL3265)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GAPDH Antibody (CL3265)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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