GAP-43 Antibody (23GB1195)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51632
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB1195
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human GAP-43 (Uniprot: P17677)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GAP-43 Antibody (23GB1195)
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (23GB1195)[NBP3-51632] -
Immunocytochemical staining of HAP-1 cells with GAP-43 antibody (1:1,000). Nuclei were stained blue with DAPI; GAP-43 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: GAP-43 Antibody (23GB1195)[NBP3-51632] -
Validation of GAP-43 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with GAP-43 antibody (1:2,000) and analyzed using CytoFLEX.
Flow Cytometry: GAP-43 Antibody (23GB1195)[NBP3-51632] -
Flow cytometric analysis of GAP-43expression in HAP-1 cells using GAP-43 antibody (1:2,000). Green, isotype control; red, Growth associated protein 43.Applications for GAP-43 Antibody (23GB1195)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GAP-43
Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.
Long Name
Growth Associated Protein 43
Alternate Names
B-50, Basp2, GAP43, Neuromodulin
Gene Symbol
GAP43
Additional GAP-43 Products
Product Documents for GAP-43 Antibody (23GB1195)
Product Specific Notices for GAP-43 Antibody (23GB1195)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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