GAP-43 Antibody (1E3) [PE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92713PE
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 1E3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
C-terminal peptide of rat and mouse GAP43 (KEDPEADQEHA) to which an N-terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Reactivity Notes
Mouse and rat.
Localization
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection > growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction > synapse. Cell projection > filopodium membrane; Peripheral membrane protein. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.
Marker
Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Applications for GAP-43 Antibody (1E3) [PE]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This GAP43 (1E3) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 43 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: GAP-43
Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.
Long Name
Growth Associated Protein 43
Alternate Names
B-50, Basp2, GAP43, Neuromodulin
Gene Symbol
GAP43
Additional GAP-43 Products
Product Documents for GAP-43 Antibody (1E3) [PE]
Product Specific Notices for GAP-43 Antibody (1E3) [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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