gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93353
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-188 of human GGCT (NP_076956.1). MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEITCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDIIKKGETQTL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images
![Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT AntibodyAzide and BSA Free [NBP2-93353]](https://resources.bio-techne.com/images/products/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Western-Blot-NBP2-93353-img0001.jpg "Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT AntibodyAzide and BSA Free [NBP2-93353]")
Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT AntibodyAzide and BSA Free [NBP2-93353]
Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP2-93353] - Analysis of extracts of various cell lines, using gamma-Glutamylcyclotransferase/CRF21/GGCT at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.Applications
Application
Recommended Usage
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: gamma-Glutamylcyclotransferase/CRF21
CRF21 (Cytochrome c-releasing factor 21; also C7orf24 or LOC79017) is a 21 kDa AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188 amino acids (aa) in length and is a complex of beta-sheets and alpha-helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70 aa substitution, while the second variant shows an 18 aa substitution for aa 97-188. Full-length human CRF21 is 82% aa identical to mouse CRF21.
Long Name
Cytochrome c Releasing Factor 21
Alternate Names
C7orf24, CRF21, gammaGlutamylcyclotransferase, GCTG, GGC, GGCT, LOC79017
Gene Symbol
GGCT
Additional gamma-Glutamylcyclotransferase/CRF21 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov