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GAD2/GAD65 Antibody (rGAD2/9382)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49117

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-49117-100ug
NBP3-49117-20ug

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # rGAD2/9382 expressed in CHO

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant human GAD2 (GAD65) protein fragment (around aa 1-200) (exact sequence is proprietary) (Uniprot: Q05329)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for GAD2/GAD65 Antibody (rGAD2/9382)

GAD2/GAD65 Antibody (rGAD2/9382)

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (rGAD2/9382) [NBP3-49117] -

Formalin-fixed, paraffin-embedded human pancreas stained with GAD2/GAD65 Antibody (rGAD2/9382). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for GAD2/GAD65 Antibody (rGAD2/9382)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Gene Symbol

GAD2

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody (rGAD2/9382)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAD2/GAD65 Antibody (rGAD2/9382)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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