Does human FGF basic show activity on mouse cells?

Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

Does the human beta-NGF polyclonal antibody (Catalog # AB-256-NA) cross-react with human BDNF, human NT-3, or mouse beta-NGF?

In Western blot, less than 5% cross-reactivity was observed with recombinant mouse β-NGF and recombinant rat β-NGF, and no cross-reactivity was observed with recombinant human (rh) NT-3 and rhNT-4.

I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

What is the heparin binding activity of FGF and VEGF?

These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

What is the specificity and cross-reactivity of Catalog # AB-233-NA?

For Western blot, less than 5% cross-reactivity was observed with recombinant human (rh) β-ECGF, and no cross-reactivity was observed with rhFGF acidic, FGF-4, FGF-5, FGF-6, FGF-7, and FGF-9.

What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors

FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Name: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-70718

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70718

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NBP1-47749

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Alexa Fluor 350

NBP2-70718AF350

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Alexa Fluor 405

NBP2-70718AF405

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Alexa Fluor 488

NBP2-70718AF488

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Alexa Fluor 532

NBP2-70718AF532

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Alexa Fluor 594

NBP2-70718AF594

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Alexa Fluor 647

NBP2-70718AF647

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Alexa Fluor 700

NBP2-70718AF700

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Alexa Fluor 750

NBP2-70718AF750

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Allophycocyanin

NBP2-70718APC

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Biotin

NBP2-70718B

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CoraFluor 1

NBP2-70718CL1

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DyLight 350

NBP2-70718UV

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DyLight 405

NBP2-70718V

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DyLight 488

NBP2-70718G

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DyLight 550

NBP2-70718R

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DyLight 594

NBP2-70718DL594

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DyLight 650

NBP2-70718C

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DyLight 680

NBP2-70718FR

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DyLight 755

NBP2-70718IR

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FITC

NBP2-70718F

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HRP

NBP2-70718H

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Janelia Fluor 525

NBP2-70718JF525

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Janelia Fluor 549

NBP2-70718JF549

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Janelia Fluor 646

NBP2-70718JF646

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Janelia Fluor 669

NBP2-70718JF669

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mFluor Violet 450 SE

NBP2-70718MFV450

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mFluor Violet 500 SE

NBP2-70718MFV500

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mFluor Violet 610 SE

NBP2-70718MFV610

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NBP2-70718PE

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PerCP

NBP2-70718PCP

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # OTI3D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free (NBP1-47749) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9)Azide and BSA Free [NBP2-70718]

Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - Analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution

Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718]

Immunocytochemistry/Immunofluorescence: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF.

Immunohistochemistry: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718]

Immunohistochemistry: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - Staining of paraffin-embedded Human pancreas tissue using anti-BFGF mouse monoclonal antibody.

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Applications for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Gene Symbol

FGF2

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews

Protocols
FAQs

FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70718

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Theoretical MW

Description

Scientific Data Images for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9)Azide and BSA Free [NBP2-70718]

Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718]

Immunohistochemistry: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718]

Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9)Azide and BSA Free [NBP2-70718]

Applications for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Western Blot

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: FGF basic/FGF2/bFGF

Long Name

Alternate Names

Gene Symbol

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free