FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70717
![Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]](https://resources.bio-techne.com/images/products/FGF-acidic-FGF1-Antibody-OTI4D2-Azide-and-BSA-Free-Western-Blot-NBP2-70717-img0002.jpg "Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4D2
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 16-155 of human AFGF (NP_000791) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
15.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free (NBP1-47748) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free
Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]
Western Blot: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF1.![Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717]](https://resources.bio-techne.com/images/products/FGF-acidic-FGF1-Antibody-OTI4D2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70717-img0003.jpg "Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717]")
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717]
Immunocytochemistry/Immunofluorescence: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGF1.![Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]](https://resources.bio-techne.com/images/products/FGF-acidic-FGF1-Antibody-OTI4D2-Azide-and-BSA-Free-Western-Blot-NBP2-70717-img0001.jpg "Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]")
Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]
Western Blot: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717] - FGF1 antibody ( 4D2 ) at 1:50000 dilution + recombinant Human FGF1.Applications for FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:5000-50000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FGF acidic/FGF1
FGF acidic/FGF1, also known as ECGF and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF1 is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF1 with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF1 can translocate to the cytosol and to the nucleus. Intracellular FGF1 functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.
Long Name
Fibroblast Growth Factor acidic
Alternate Names
alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1
Gene Symbol
FGF1
Additional FGF acidic/FGF1 Products
Product Documents for FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free
Product Specific Notices for FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...