Ferritin Heavy Chain Antibody (23GB1835)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51989

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NBP3-51989-100ul
NBP3-51989-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1835

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ferritin Heavy Chain Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Ferritin Heavy Chain (Uniprot: P02794)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ferritin Heavy Chain Antibody (23GB1835)

Ferritin Heavy Chain Antibody (23GB1835)

Immunocytochemistry/ Immunofluorescence: Ferritin Heavy Chain Antibody (23GB1835)[NBP3-51989] -

Immunocytochemical staining of HepG2 cells with Ferritin Heavy Chain antibody (1:1,000). Nuclei were stained blue with DAPI; Ferritin heavy chain 1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Ferritin Heavy Chain Antibody (23GB1835)

Flow Cytometry: Ferritin Heavy Chain Antibody (23GB1835)[NBP3-51989] -

Flow cytometric analysis of Ferritin Heavy Chainexpression in HepG2 cells using Ferritin Heavy Chain antibody (1:2,000). Green, isotype control; red, Ferritin heavy chain 1.
Ferritin Heavy Chain Antibody (23GB1835)

Western Blot: Ferritin Heavy Chain Antibody (23GB1835)[NBP3-51989] -

Western blotting analysis using Ferritin Heavy Chain antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Ferritin Heavy Chain Antibody (23GB1835)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ferritin Heavy Chain

Stores iron in a soluble. nontoxic. readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy; 21 kDa) and L (light; 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.

Alternate Names

apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, ferritin heavy chain, ferritin, heavy polypeptide 1, FHC, FTHEC 1.16.3.1, FTHL6MGC104426, PIG15, placenta immunoregulatory factor, PLIF, proliferation-inducing protein 15

Gene Symbol

FTH1

Additional Ferritin Heavy Chain Products

Product Documents for Ferritin Heavy Chain Antibody (23GB1835)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ferritin Heavy Chain Antibody (23GB1835)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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