Fas Ligand/TNFSF6 Antibody (14C2) [DyLight 350]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-63072UV
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunoprecipitation, Western Blot (Negative)
Label
DyLight 350 (Excitation = 353 nm, Emission = 432 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 14C2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Made to Human CD178
Localization
Cell membrane: single pass type II membrane protein. Secreted protein: may be released into the extracellular fluid, probably by cleavage form the cell surface.
Specificity
NB100-63072 recognizes the human CD178 cell surface antigen, a 40kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis. Clone 14C2 is not reported to have a blocking function.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for Fas Ligand/TNFSF6 Antibody (14C2) [DyLight 350]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot (Negative)
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Results maybe enhanced using membrane permeabilisation in Flow Cytometry.
Recommended applications are based on validated applications from the unconjugated base product NB100-63072. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Recommended applications are based on validated applications from the unconjugated base product NB100-63072. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
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Plan Your Experiments
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Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Fas Ligand/TNFSF6
Alternate Names
CD178, CD95L, FASLG, TNFSF6
Gene Symbol
FASLG
Additional Fas Ligand/TNFSF6 Products
Product Documents for Fas Ligand/TNFSF6 Antibody (14C2) [DyLight 350]
Product Specific Notices for Fas Ligand/TNFSF6 Antibody (14C2) [DyLight 350]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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