FAM105B/OTULIN Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14722
![Western Blot: FAM105B/OTULIN Antibody [NBP2-14722]](https://resources.bio-techne.com/images/products/FAM105B-OTULIN-Antibody-Western-Blot-NBP2-14722-img0009.jpg "Western Blot: FAM105B/OTULIN Antibody [NBP2-14722]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: DEIEKEKELLIHERGASEPRLSVAPEMDIMDYCKKEWRGNTQKATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQAMSQAVGLPPWLQDPELMLLP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FAM105B/OTULIN Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: FAM105B/OTULIN Antibody [NBP2-14722]](https://resources.bio-techne.com/images/products/FAM105B-OTULIN-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14722-img0005.jpg "Immunocytochemistry/ Immunofluorescence: FAM105B/OTULIN Antibody [NBP2-14722]")
Immunocytochemistry/ Immunofluorescence: FAM105B/OTULIN Antibody [NBP2-14722]
Immunocytochemistry/Immunofluorescence: FAM105B/OTULIN Antibody [NBP2-14722] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & mitochondria.![Immunohistochemistry-Paraffin: FAM105B/OTULIN Antibody [NBP2-14722]](https://resources.bio-techne.com/images/products/FAM105B-OTULIN-Antibody-Immunohistochemistry-Paraffin-NBP2-14722-img0001.jpg "Immunohistochemistry-Paraffin: FAM105B/OTULIN Antibody [NBP2-14722]")
Immunohistochemistry-Paraffin: FAM105B/OTULIN Antibody [NBP2-14722]
Immunohistochemistry-Paraffin: FAM105B/OTULIN Antibody [NBP2-14722] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Applications for FAM105B/OTULIN Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FAM105B/OTULIN
Family with Sequence Similarity 105, Member B (FAM105B), also known as OTULIN, is a 352 amino acid (aa) member of the ovarian tumor protein superfamily with a predicted molecular weight of 40 kDa. The mouse FAM105B/OTULIN ortholog shares 89% aa sequence identity with the human protein. It is a C65 type peptidase that has high specificity for methionine-1-linked linear Ubiquitin chains and contributes to the regulation of NFkB signaling.
Long Name
Family with Sequence Similarity 105, Member B
Alternate Names
OTULIN
Gene Symbol
OTULIN
Additional FAM105B/OTULIN Products
Product Documents for FAM105B/OTULIN Antibody - BSA Free
Product Specific Notices for FAM105B/OTULIN Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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