Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A3

Concentration

1 mg/ml

View all ETS2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 90-349 of human ETS2(NP_005230) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse ETS2 Antibody (OTI2A3) (NBP2-45543) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ETS2 Antibody (OTI2A3)

Western Blot: ETS2 Antibody (OTI2A3) [NBP2-45543]

Western Blot: ETS2 Antibody (OTI2A3) [NBP2-45543]

Western Blot: ETS2 Antibody (OTI2A3) [NBP2-45543] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETS2.
Immunohistochemistry: ETS2 Antibody (OTI2A3) [NBP2-45543]

Immunohistochemistry: ETS2 Antibody (OTI2A3) [NBP2-45543]

Immunohistochemistry: ETS2 Antibody (OTI2A3) [NBP2-45543] - Analysis of Carcinoma of Human thyroid tissue.
Western Blot: ETS2 Antibody (OTI2A3) [NBP2-45543]

Western Blot: ETS2 Antibody (OTI2A3) [NBP2-45543]

Western Blot: ETS2 Antibody (OTI2A3) [NBP2-45543] - Analysis of extracts (10ug) from 2 different cell lines.
View 8 more images

Applications for ETS2 Antibody (OTI2A3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ETS2

ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Alternate Names

ETS2IT1, human erythroblastosis virus oncogene homolog 210protein C-ets-2, oncogene ETS-2, v-ets avian erythroblastosis virus E2 oncogene homolog 2, v-ets avian erythroblastosis virus E26 oncogene homolog 2, v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)

Gene Symbol

ETS2

Additional ETS2 Products

Product Documents for ETS2 Antibody (OTI2A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ETS2 Antibody (OTI2A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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