ENC1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-46571

Novus Biologicals, part of Bio-Techne
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NBP3-46571
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ENC1 Antibodies »

Product Specifications

Immunogen

ectodermal-neural cortex(with BTB-like domain). (Uniprot# O14682)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ENC1 Antibody - BSA Free

ENC1 Antibody - BSA Free

Immunoprecipitation: ENC1 Antibody - BSA Free [NBP3-46571] -

Immunoprecipitation: ENC1 Antibody - BSA Free [NBP3-46571] - IP Result of anti-ENC1 (IP:, 4ug; Detection: 1:1000) with mouse brain tissue lysate 2640ug.
ENC1 Antibody - BSA Free

Western Blot: ENC1 Antibody - BSA Free [NBP3-46571] -

Western Blot: ENC1 Antibody - BSA Free [NBP3-46571] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (ENC1 antibody) at dilution of 1:400
ENC1 Antibody - BSA Free

Immunohistochemistry: ENC1 Antibody - BSA Free [NBP3-46571] -

Immunohistochemistry: ENC1 Antibody - BSA Free [NBP3-46571] - Immunohistochemistry of paraffin-embedded human pancreas using (ENC1 antibody) at dilution of 1:50

Applications for ENC1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
Theoretical Molecular Weight is 67 kDa, 57 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ENC1

DNA damage and/or hyperproliferative signals activate wildtype p53 tumor suppressor protein (TP53; MIM 191170), inducing cell cycle arrest or apoptosis. Mutations that inactivate p53 occur in 50% of all tumors. Polyak et al. (1997) [PubMed 9305847] used serial analysis of gene expression (SAGE) to evaluate cellular mRNA levels in a colorectal cancer cell line transfected with p53. Of 7,202 transcripts identified, only 14 were expressed at levels more than 10-fold higher in p53-expressing cells than in control cells. Polyak et al. (1997) [PubMed 9305847] termed these genes 'p53-induced genes,' or PIGs, several of which were predicted to encode redox-controlling proteins. They noted that reactive oxygen species (ROS) are potent inducers of apoptosis. Flow cytometric analysis showed that p53 expression induces ROS production, which increases as apoptosis progresses under some conditions. The authors stated that the PIG10 gene, also called ENC1, encodes an actin-binding protein.[supplied by OMIM]

Alternate Names

CCL28, ectodermal-neural cortex 1 (with BTB-like domain), ENC-1BTB domain-like (brain), FLJ39259, PIG10KLHL35, tumor protein p53 inducible protein 10

Gene Symbol

ENC1

Additional ENC1 Products

Product Documents for ENC1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ENC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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