Elk-1 Antibody (10U8X1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15631
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10U8X1 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 329-428 of human Elk-1 (NP_001107595.1). GGPGPERTPGSGSGSGLQAPGPALTPSLLPTHTLTPVLLTPSSLPPSIHFWSTLSPIAPRSPAKLSFQFPSSGSAQVHIPSISVDGLSTPVVLSPGPQKP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Elk-1 Antibody (10U8X1) (NBP3-15631) is a recombinant monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Elk-1 Antibody (10U8X1)
![Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631]](https://resources.bio-techne.com/images/products/Elk-1-Antibody-10U8X1-Western-Blot-NBP3-15631-img0001.jpg "Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631]")
Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631]
Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631] - Western blot analysis of extracts of various cell lines, using Elk-1 antibody (NBP3-15631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.![Immunoprecipitation: Elk-1 Antibody (10U8X1) [NBP3-15631]](https://resources.bio-techne.com/images/products/Elk-1-Antibody-10U8X1-Immunoprecipitation-NBP3-15631-img0002.jpg "Immunoprecipitation: Elk-1 Antibody (10U8X1) [NBP3-15631]")
Immunoprecipitation: Elk-1 Antibody (10U8X1) [NBP3-15631]
Immunoprecipitation: Elk-1 Antibody (10U8X1) [NBP3-15631] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Elk-1 antibody (NBP3-15631). Western blot was performed from the immunoprecipitate using Elk-1 antibody (NBP3-15631) at a dilition of 1:1000.Applications for Elk-1 Antibody (10U8X1)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Elk-1
Alternate Names
Elk1
Gene Symbol
ELK1
Additional Elk-1 Products
Product Documents for Elk-1 Antibody (10U8X1)
Product Specific Notices for Elk-1 Antibody (10U8X1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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