EGFR Antibody (ICR10) [PE]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-64131
![EGFR Antibody (ICR10) [PE] Flow Cytometry: EGFR Antibody (ICR10) [PE] - [NB100-64131] -](https://resources.bio-techne.com/images/products/nb100-64131_rat-monoclonal-egfr-antibody-icr10-pe-231202523584716.jpg "Flow Cytometry: EGFR Antibody (ICR10) [PE] - [NB100-64131] -")
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Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # ICR10
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Extracellular domain of human EGF-receptor from head and neck carcinoma
Epitope
ICR10 binds to epitope B, and has an affinity of 6.7x10-9 M.
Localization
Cell Membrane and Secreted
Specificity
EGFR. It does not react with erb-B2.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for EGFR Antibody (ICR10) [PE]
Flow Cytometry: EGFR Antibody (ICR10) [PE] - [NB100-64131] -
Flow Cytometry: EGFR Antibody (ICR10) [PE] - [NB100-64131] - Figure A. PE conjugated Mouse anti Human CD49c and Alexa Fluor 647 conjugated Rat IgG2a isotype control Figure B . PE conjugated Rat anti Human CD49c and Alexa Fluor 647 conjugated Mouse anti Human EGFR All experiments performed on live single A431 cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell AnalyzerApplications for EGFR Antibody (ICR10) [PE]
Application
Recommended Usage
Flow Cytometry
Neat
Application Notes
For Flow Cytometry, use 10 uL of the suggested working dilution to label 10^6 cells in 100 uL.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Reconstitute with 1.0 mL deionized water.
Formulation
PBS, 1% Bovine Serum Albumin, 5% Sucrose
Preservative
0.09% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: EGFR
In addition to its role in normal development, EGFR mutations or overexpression is observed in many tumors, including breast cancer, non-small cell lung carcinoma (NSCLC), colon cancer, and more (3-6). Small molecule tyrosine kinase inhibitors (TKIs), like gefitinib, erlotinib, and afatinib, have shown great efficacy in treating patients with EGFR activating mutations, especially for NSCLC (4-6). However, most patients eventually develop acquired resistance to TKIs and thus combination and alternative therapies are in development (4-6). A third-generation TKI, osimertinib, is approved for NSCLC patients with resistance to first-line EGFR TKI treatment (6). Additionally, combination therapies of EGFR TKIs with monoclonal antibody immunotherapies, like anti-PD-L1, are being further investigated in clinical trials (6).
References
1. Roskoski R Jr. Small molecule inhibitors targeting the EGFR/ErbB family of protein-tyrosine kinases in human cancers. Pharmacol Res. 2019; 139:395-411. https://doi.org/10.1016/j.phrs.2018.11.014
2. Sigismund S, Avanzato D, Lanzetti L. Emerging functions of the EGFR in cancer. Mol Oncol. 2018; 12(1):3-20. https://doi.org/10.1002/1878-0261.12155
3. Normanno N, De Luca A, Bianco C, et al. Epidermal growth factor receptor (EGFR) signaling in cancer. Gene. 2006; 366(1):2-16. https://doi.org/10.1016/j.gene.2005.10.018
4. Liu Q, Yu S, Zhao W, Qin S, Chu Q, Wu K. EGFR-TKIs resistance via EGFR-independent signaling pathways. Mol Cancer. 2018; 17(1):53. https://doi.org/10.1186/s12943-018-0793-1
5. Harrison PT, Vyse S, Huang PH. Rare epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer. Semin Cancer Biol. 2020; 61:167-179. https://doi.org/10.1016/j.semcancer.2019.09.015
6. Wu SG, Shih JY. Management of acquired resistance to EGFR TKI-targeted therapy in advanced non-small cell lung cancer. Mol Cancer. 2018; 17(1):38. https://doi.org/10.1186/s12943-018-0777-1
Long Name
Epidermal Growth Factor Receptor
Alternate Names
EGF R, ErbB, ErbB1, HER-1
Gene Symbol
EGFR
UniProt
Additional EGFR Products
Product Documents for EGFR Antibody (ICR10) [PE]
Product Specific Notices for EGFR Antibody (ICR10) [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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