EBAG9/RCAS1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00242
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human EBAG9/RCAS1 (Accession#: O00559; Arg28-Ser213)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for EBAG9/RCAS1 Antibody - Azide and BSA Free
Western Blot: EBAG9/RCAS1 Antibody [NBP3-00242] -
Western Blot: EBAG9/RCAS1 Antibody [NBP3-00242] - NBP3-00242 at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: THP1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:24 kDa Observed band size:37 kDa
Immunohistochemistry-Paraffin: EBAG9/RCAS1/RCAS1 Antibody [NBP3-00242] -
Immunohistochemistry-Paraffin: EBAG9/RCAS1/RCAS1 Antibody [NBP3-00242] - Immunochemical staining of human EBAG9/RCAS1 in human skeletal muscle with NBP3-00242 (1:2000, formalin-fixed paraffin embedded sections).
Immunoprecipitation: EBAG9/RCAS1 Antibody [NBP3-00242] -
Immunoprecipitation: EBAG9/RCAS1 Antibody [NBP3-00242] - EBAG9 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 uL NBP3-00242 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00242, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size: 37 kDaApplications for EBAG9/RCAS1 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry-Paraffin
1:1000-1:4000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EBAG9/RCAS1
Alternate Names
Cancer-associated surface antigen RCAS1, EB9cancer associated surface antigen, estrogen receptor binding site associated, antigen, 9, Estrogen receptor-binding fragment-associated gene 9 protein, RCAS1PDAF, receptor-binding cancer antigen expressed on SiSo cells
Gene Symbol
EBAG9
Additional EBAG9/RCAS1 Products
Product Documents for EBAG9/RCAS1 Antibody - Azide and BSA Free
Product Specific Notices for EBAG9/RCAS1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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