E4F1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56386
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: RHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQM
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit E4F1 Antibody - BSA Free (NBP2-56386) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for E4F1 Antibody - BSA Free
![Western Blot: E4F1 Antibody [NBP2-56386]](https://resources.bio-techne.com/images/products/E4F1-Antibody-Western-Blot-NBP2-56386-img0002.jpg "Western Blot: E4F1 Antibody [NBP2-56386]")
Western Blot: E4F1 Antibody [NBP2-56386]
Western Blot: E4F1 Antibody [NBP2-56386] - Analysis in human cell line RT-4 and human cell line U-251 MG.![Immunocytochemistry/ Immunofluorescence: E4F1 Antibody [NBP2-56386]](https://resources.bio-techne.com/images/products/E4F1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56386-img0001.jpg "Immunocytochemistry/ Immunofluorescence: E4F1 Antibody [NBP2-56386]")
Immunocytochemistry/ Immunofluorescence: E4F1 Antibody [NBP2-56386]
Immunocytochemistry/Immunofluorescence: E4F1 Antibody [NBP2-56386] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.![E4F1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: E4F1 Antibody - BSA Free [NBP2-56386]](https://resources.bio-techne.com/images/products/nbp2-56386_rabbit-polyclonal-e4f1-antibody-25620259114032.jpg "Chromatin Immunoprecipitation-exo-Seq: E4F1 Antibody - BSA Free [NBP2-56386]")
Chromatin Immunoprecipitation-exo-Seq: E4F1 Antibody - BSA Free [NBP2-56386]
ChIP-Exo-Seq composite graph for Anti-E4F1 (NBP2-56386) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for E4F1 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: E4F1
Alternate Names
E4F transcription factor 1p50E4F, E4FMGC99614, EC 6.3.2.-, p120E4F, Putative E3 ubiquitin-protein ligase E4F1, Transcription factor E4F, transcription factor E4F1
Gene Symbol
E4F1
Additional E4F1 Products
Product Documents for E4F1 Antibody - BSA Free
Product Specific Notices for E4F1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...