dsRNA Antibody (K2) Best Seller
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11398
Key Product Details
Species Reactivity
Multi-Species
Applications
ELISA, Immunofluorescence, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # K2
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.
Specificity
This antibody recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp dsRNA. Recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by this antibody.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
After delivery antibodies should be aliquoted and stored at -20 or -70C. After adding 10 mM sodium azide undiluted antibody can also be stored at 4C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. This ds-RNA antibody was manufactured by Scicon.
Applications for dsRNA Antibody (K2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is primarily used for a sandwich ELISA to detect and quantitate (after calibration) dsRNA (see Schonborn et al.). For this application it should be diluted 1:2 with PBS. It may also be advantageous to use it for immunofluorescence studies. The optimum working dilution of the antibody for any specific application should be established by titration.
Not for use for clinical purposes. For in vitro use only.
dsRNA-immunoblotting.
Not for use for clinical purposes. For in vitro use only.
dsRNA-immunoblotting.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Culture supernatant (RPMI, 5% fetal calf serum)
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: dsRNA
Alternate Names
Double-stranded RNA
Additional dsRNA Products
Product Documents for dsRNA Antibody (K2)
Product Specific Notices for dsRNA Antibody (K2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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