DNA Polymerase epsilon catalytic subunit A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13067
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (94%), Rat (90%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human DNA Polymerase epsilon catalytic subunit A. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
262 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for DNA Polymerase epsilon catalytic subunit A Antibody
Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067]
Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - DNA Polymerase epsilon catalytic subunit A antibody detects DNA Polymerase epsilon catalytic subunit A protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minWestern Blot: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] -
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody (NBP3-13067) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Immunocytochemistry/ Immunofluorescence: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] -
POLE antibody detects POLE protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: POLE protein stained by POLE antibody (NBP3-13067) diluted at 1:2500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Applications for DNA Polymerase epsilon catalytic subunit A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:2500
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNA Polymerase epsilon catalytic subunit A
Alternate Names
DKFZp434F222, DNA polymerase epsilon catalytic subunit A, DNA polymerase II subunit A, EC 2.7.7, EC 2.7.7.7, POLE1FLJ21434, polymerase (DNA directed), epsilon
Gene Symbol
POLE
Additional DNA Polymerase epsilon catalytic subunit A Products
Product Documents for DNA Polymerase epsilon catalytic subunit A Antibody
Product Specific Notices for DNA Polymerase epsilon catalytic subunit A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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