DHX15 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-586
![Western Blot: DHX15 Antibody [NB100-586]](https://resources.bio-techne.com/images/products/DHX15-Antibody-Western-Blot-NB100-586-img0006.jpg "Western Blot: DHX15 Antibody [NB100-586]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 795) of human DEAH box polypeptide 15 using the numbering given in Swiss-Prot entry O43143 (GeneID 1665).
Specificity
NB100-586 is specific for human DHX 15 protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DHX15 Antibody - BSA Free
Western Blot: DHX15 Antibody [NB100-586]
Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody NB100-586 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Western Blot: DHX15 Antibody [NB100-586]](https://resources.bio-techne.com/images/products/DHX15-Antibody-Western-Blot-NB100-586-img0002.jpg "Western Blot: DHX15 Antibody [NB100-586]")
Western Blot: DHX15 Antibody [NB100-586]
Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 on HeLa whole cell lysate using NB100-586. Immunoprecipitated DHX15 was blotted using NB100-585 at 0.1 mcg/ml. NB100-585 was also used unsuccessfully for IP.Applications for DHX15 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1-5 ug/mg lysate
Western Blot
1:5000-1:15000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000 - 1:15,000 Immunoprecipitation: 1 to 5 ug/mg lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DHX15
Alternate Names
ATP-dependent RNA helicase #46, DBP1DEAH box protein 15, DDX15putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15, DEAH (Asp-Glu-Ala-His) box polypeptide 15, EC 3.6.1, EC 3.6.4.13, HRH2DEAD/H box-15, PRP43, PRPF43, PrPp43p, RNA helicase 2
Entrez Gene IDs
1665 (Human)
Gene Symbol
DHX15
UniProt
Additional DHX15 Products
Product Documents for DHX15 Antibody - BSA Free
Product Specific Notices for DHX15 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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