DDX52 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33776
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KFDTRRFSADAARFQIGKRKYDFDSSEVLQGLDFFGNKKSVPGVCGASQTHQKPQNGEKKEESLTERKREQSKKKRKTMTSEIAS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DDX52 Antibody - BSA Free
Immunohistochemistry-Paraffin: DDX52 Antibody [NBP2-33776]
Immunohistochemistry-Paraffin: DDX52 Antibody [NBP2-33776] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.Western Blot: DDX52 Antibody - BSA Free [NBP2-33776] -
c-Myc regulates DDX52 expression in PCa. a, b 22RV1 and PC3 cells were infected with lentiviruses carrying shRNA against DDX52, c-Myc or shCON, and gene expression was determined using western blotting. c Published PCa datasets were analyzed to assess the correlation between mRNA expression of DDX52 and c-Myc. f Representative photographs of PCa tissues stained with DDX52 and c-Myc (scale bar: 100 μm). e Correlation between the expression levels of DDX52 and c-Myc as determined using the IHC score (n = 86) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34399732), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: DDX52 Antibody - BSA Free [NBP2-33776] -
DDX52 knockdown inhibits PCa xenograft growth. a The mice were killed at 6 weeks after implanting 22RV1 cells, and the volumes of the xenograft tumors are shown. b Weights of the xenograft tumors in the control and treated groups (Mann–Whitney test, n = 7, error bars represent standard deviations; ***p < 0.001). c Kaplan–Meier plot showing the time of xenograft genesis after implantation (log-rank test; ***p < 0.001). d Examples of tumor xenografts that underwent hematoxylin and eosin (HE) staining and DDX52 and Ki67 immunohistochemical staining (scale bar: 100 μm). e, f The positive cells in random areas in each xenograft section were counted. e DDX52 expression was measured according to the IHC score (Mann–Whitney test, error bars represent standard deviations; **p < 0.01). f Ki67 expression was measured according to the number of positively stained cells (Mann–Whitney test, error bars represent standard deviations; **p < 0.01) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34399732), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for DDX52 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
Use in WB reported in scientific literature (PMID:34399732). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DDX52
Alternate Names
ATP-dependent RNA helicase ROK1-like, DEAD (Asp-Glu-Ala-Asp) box polypeptide 52, DEAD box protein 52, EC 3.6.1, HUSSY19, probable ATP-dependent RNA helicase DDX52, ROK1EC 3.6.4.13
Gene Symbol
DDX52
UniProt
Additional DDX52 Products
Product Documents for DDX52 Antibody - BSA Free
Product Specific Notices for DDX52 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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