DDX1 Antibody (3E5E2) - BSA Free

Western Blot Shows DDX1 Specificity Using Knockout Cell Line.

Western blot shows lysates of U2OS human osteosarcoma parental cell line and DDX1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with DDX1 Antibody (3E5E2) - BSA Free (Catalog # NBP2-61745) followed by HRP-conjugated secondary antibody. A specific band was detected for DDX1 at approximately 82.4 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of DDX1 by Immunoprecipitation.

U2OS human osteosarcoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of DDX1 Antibody (3E5E2) - BSA Free (Catalog # NBP2-61745) pre-coupled to Dynabeads Protein G. Immunoprecipitated DDX1 was detected in Western Blot with a rabbit DDX1 antibody used at 1/1000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot: DDX1 Antibody (3E5E2)BSA Free [NBP2-61745]

Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.