DARS Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86027

Novus Biologicals, part of Bio-Techne
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NBP1-86027
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DARS Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LHVQKIYVISLAEPRLPLQLDDAVRPEAEGEEEGRATVNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICAD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DARS Antibody - BSA Free

Western Blot: DARS Antibody [NBP1-86027]

Western Blot: DARS Antibody [NBP1-86027]

Western Blot: DARS Antibody [NBP1-86027] - Analysis using Anti-DARS antibody NBP1-86027 (A) shows similar pattern to independent antibody NBP1-85938 (B).
Immunocytochemistry/ Immunofluorescence: DARS Antibody [NBP1-86027]

Immunocytochemistry/ Immunofluorescence: DARS Antibody [NBP1-86027]

Immunocytochemistry/Immunofluorescence: DARS Antibody [NBP1-86027] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
DARS Antibody - BSA Free Immunohistochemistry-Paraffin: DARS Antibody - BSA Free [NBP1-86027]

Immunohistochemistry-Paraffin: DARS Antibody - BSA Free [NBP1-86027]

Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and cells in glomeruli.
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Applications for DARS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DARS

Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.

Alternate Names

aspartate tRNA ligase 1, cytoplasmic, Aspartate--tRNA ligase, aspartyl-tRNA synthetase, aspartyl-tRNA synthetase, cytoplasmic, AspRS, Cell proliferation-inducing gene 40 protein, cell proliferation-inducing protein 40, DKFZp781B11202, EC 6.1.1.12, MGC111579

Gene Symbol

DARS1

Additional DARS Products

Product Documents for DARS Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DARS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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