Cytokeratin, Basic (Type II or HMW) Antibody (SPM591) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48220
![Immunohistochemistry-Paraffin: Cytokeratin, Basic (Type II or HMW) Antibody (SPM591) - IHC-Prediluted [NBP2-48220]](https://resources.bio-techne.com/images/products/Cytokeratin-Basic-Type-II-or-HMW-Antibody-SPM591-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48220-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, Basic (Type II or HMW) Antibody (SPM591) - IHC-Prediluted [NBP2-48220]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM591
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Solubilized keratin extract from human stratum corneum
Localization
Cytoplasmic
Specificity
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This monoclonal antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images
Immunohistochemistry-Paraffin: Cytokeratin, Basic (Type II or HMW) Antibody (SPM591) - IHC-Prediluted [NBP2-48220]
Immunohistochemistry-Paraffin: Cytokeratin, Basic (Type II or HMW) Antibody (SPM591) - IHC-Prediluted [NBP2-48220] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (SPM591).Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin, Basic (Type II or HMW)
Alternate Names
CK1, CK10, CK14, CK5, cytokeratin 1, cytokeratin 10, cytokeratin 14, cytokeratin 5
Additional Cytokeratin, Basic (Type II or HMW) Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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