Cytokeratin, Basic (Type II or HMW) Antibody (SPM116)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34348
![Immunohistochemistry-Paraffin: Cytokeratin, Basic (Type II or HMW) Antibody (SPM116) [NBP2-34348]](https://resources.bio-techne.com/images/products/Cytokeratin-Basic-Type-II-or-HMW-Antibody-SPM116-Immunohistochemistry-Paraffin-NBP2-34348-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, Basic (Type II or HMW) Antibody (SPM116) [NBP2-34348]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM116
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human epidermal keratin (Uniprot: Q01546)
Localization
Cytoplasmic
Specificity
This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. This monoclonal antibody recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34395)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin, Basic (Type II or HMW) Antibody (SPM116)
Immunohistochemistry-Paraffin: Cytokeratin, Basic (Type II or HMW) Antibody (SPM116) [NBP2-34348]
Immunohistochemistry-Paraffin: Cytokeratin, Basic (Type II or HMW) Antibody (SPM116) [NBP2-34348] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with cytokeratin, HMW Monoclonal Antibody (SPM116)Applications for Cytokeratin, Basic (Type II or HMW) Antibody (SPM116)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin, Basic (Type II or HMW)
Alternate Names
CK1, CK10, CK14, CK5, cytokeratin 1, cytokeratin 10, cytokeratin 14, cytokeratin 5
UniProt
Additional Cytokeratin, Basic (Type II or HMW) Products
Product Documents for Cytokeratin, Basic (Type II or HMW) Antibody (SPM116)
Product Specific Notices for Cytokeratin, Basic (Type II or HMW) Antibody (SPM116)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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