Cytokeratin 8 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16094
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Zebrafish
Cited:
Human
Predicted:
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Validated:
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%), Porcine (83%), Bovine (84%), Canine (89%).
Localization
Cytoplasm
Marker
Epithelial Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 8 Antibody - BSA Free
![Western Blot: Cytokeratin 8 Antibody [NBP2-16094]](https://resources.bio-techne.com/images/products/Cytokeratin-8-Antibody-Western-Blot-NBP2-16094-img0009.jpg "Western Blot: Cytokeratin 8 Antibody [NBP2-16094]")
Western Blot: Cytokeratin 8 Antibody [NBP2-16094]
Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094]](https://resources.bio-techne.com/images/products/Cytokeratin-8-Antibody-Immunohistochemistry-Paraffin-NBP2-16094-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094]")
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094]
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094] - Gastric ca, using Cytokeratin 8 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: Cytokeratin 8 Antibody [NBP2-16094]](https://resources.bio-techne.com/images/products/Cytokeratin-8-Antibody-Western-Blot-NBP2-16094-img0002.jpg "Western Blot: Cytokeratin 8 Antibody [NBP2-16094]")
Western Blot: Cytokeratin 8 Antibody [NBP2-16094]
Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.
Applications for Cytokeratin 8 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry Whole-Mount
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:5000-1:20000
Reviewed Applications
Read 1 review rated 5 using NBP2-16094 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin 8
Additional Cytokeratin 8 Products
Product Documents for Cytokeratin 8 Antibody - BSA Free
Product Specific Notices for Cytokeratin 8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.