Cytokeratin 8 Antibody (KRT8/2174R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49455
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Porcine, Bovine, Rabbit, Sheep
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # KRT8/2174R
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human KRT8 protein (Uniprot: P05787)
Localization
Cytoplasm, Nucleoplasm, Nucleus, Nucleus matrix
Specificity
Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Scientific Data Images for Cytokeratin 8 Antibody (KRT8/2174R) - Azide and BSA Free
Western Blot: Cytokeratin 8 Antibody (KRT8/2174R) - Azide and BSA Free [NBP3-49455] -
Western Blot analysis of HCT116 cell lysate using Cytokeratin 8 Antibody (KRT8/2174R) - Azide and BSA Free.Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (KRT8/2174R) - Azide and BSA Free [NBP3-49455] -
Formalin-fixed, paraffin-embedded human Small Intestine stained with Cytokeratin 8 Antibody (KRT8/2174R) - Azide and BSA Free.Applications for Cytokeratin 8 Antibody (KRT8/2174R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin 8
Alternate Names
CARD2, CK8, EndoA, KRT8
Gene Symbol
KRT8
Additional Cytokeratin 8 Products
Product Documents for Cytokeratin 8 Antibody (KRT8/2174R) - Azide and BSA Free
Product Specific Notices for Cytokeratin 8 Antibody (KRT8/2174R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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