Cytokeratin 8/18 Antibody (C-51) [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11565H
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Porcine, Bovine, Chicken (Negative), Donkey (Negative), Ferret (Negative), Monkey, Mouse (Negative), Rabbit (Negative), Sheep, Xenopus (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # C-51
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Cytoskeleton preparation from HeLa cells
Reactivity Notes
Does not react with Mouse, Ferret, Rabbit, donkey, Chicken or Xenopus laevis.
Rat reactivity reported in scientific liteRature (PMID: 28975767).
Rat reactivity reported in scientific liteRature (PMID: 28975767).
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for Cytokeratin 8/18 Antibody (C-51) [HRP]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Cytokeratin 8/18
Alternate Names
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, Keratin-8, KO, Type-II keratin Kb8
Gene Symbol
KRT8
Additional Cytokeratin 8/18 Products
Product Documents for Cytokeratin 8/18 Antibody (C-51) [HRP]
Product Specific Notices for Cytokeratin 8/18 Antibody (C-51) [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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