Cytokeratin 7/13/17/18 Antibody (ST50-05)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67533

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-67533
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST50-05 expressed in HEK293

Concentration

1 mg/ml

View all Cytokeratin 7/13/17/18 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Cytokeratin 7/13/17/18 aa 18-67. (SwissProt: P08729 Human; SwissProt: Q9DCV7 Mouse)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Cytokeratin 7/13/17/18 Antibody (ST50-05) (NBP2-67533) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Cytokeratin 7/13/17/18 Antibody (ST50-05)

Western Blot: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Western Blot: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Western Blot: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of Cytokeratin 7 on different lysates using anti-Cytokeratin 7 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: HepG2 Lane 3: SK-BR-3 Lane 4: SW1990
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of SKBR-3 cells labeling Cytokeratin 7. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS for 10 minutes and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with the primary antibodies Cytokeratin 7 (red) at 1/100 dilution for overnight at 4 . Goat anti rabbit IgG (iFluorTM 594) was used as the secondary antibody at 1/1,000 dilution dilution. DAPI was used as nuclear counterstain.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 ( washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
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Applications for Cytokeratin 7/13/17/18 Antibody (ST50-05)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:400

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 7/13/17/18

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue- and differentiation-specific expression profiles. Keratins 7 and 8 are two closely related type II keratins characteristic of simple epithelia. Keratin 7 is less widespread than keratin 8 and is expressed in sebaceous and sweat glands and some cells of the inner hair root sheath. Keratin 7 is often co-expressed with keratin 19. Keratin 17 is expressed in suprabasal keratinocytes of wounded epidermis. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. LdS 23 is useful for studies of epithelial cells and tumour origin.

Alternate Names

CK7, CK-7, cytokeratin 7, cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, Keratin-7, MGC129731, MGC3625, sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Gene Symbol

KRT7

Additional Cytokeratin 7/13/17/18 Products

Product Documents for Cytokeratin 7/13/17/18 Antibody (ST50-05)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytokeratin 7/13/17/18 Antibody (ST50-05)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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