Cytokeratin 20 Antibody (CL9390) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88932

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9390

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 20 Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P35900, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytokeratin 20 Antibody (CL9390) - BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932] - Analysis in human rectum and prostate tissues using NBP2-88932 antibody. Corresponding KRT20 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (CL9390) [NBP2-88932] - Staining of human kidney shows no positivity in cells in tubules as expected.
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Applications for Cytokeratin 20 Antibody (CL9390) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 20

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Alternate Names

CD20, CK20, CK-20, Cytokeratin-20, K20cytokeratin 20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT21, MGC35423, Protein IT

Gene Symbol

KRT20

Additional Cytokeratin 20 Products

Product Documents for Cytokeratin 20 Antibody (CL9390) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 20 Antibody (CL9390) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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