Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60776
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Guinea Pig (100%), Hamster (100%), Primate (100%), Rabbit (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr153 conjugated to KLH. Accession # P12532
Reactivity Notes
This antibody detects both Isoform 1 and 2 of the protein.
Modification
p Tyr153
Specificity
Specific for endogenous levels of the ~46 kDa Creatine kinase MT 1A protein phosphorylated at Tyr153. Immunolabeling of Creatine kinase MT 1A is greatly decreased by lambda-phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free (NBP2-60776) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free
![Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776]](https://resources.bio-techne.com/images/products/Creatine-kinase-MT-1A-[p-Tyr153]-Antibody-Western-Blot-NBP2-60776-img0001.jpg "Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776]")
Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776]
Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776] - Western blot of mouse mitochondria from whole brain lysateshowing specific immunolabeling of the ~46 kDa CK1Mt proteinphosphorylated at Tyr153 in the first lane (-). Phosphospecificityis shown in the second lane (+) where the immunolabeling isgreatly decreased by blot treatment with lambda phosphatase(Lambda-Ptase, 1200 units for 30 minutes).Applications for Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free
Application
Recommended Usage
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
Format
Azide Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Creatine kinase MT 1A
Alternate Names
Acidic-Type Mitochondrial Creatine Kinase, CKMT, CKMT1, CKMT1A, Creatine Kinase U-Type, Mitochondrial, Creatine Kinase, Mitochondrial 1 (Ubiquitous), Creatine Kinase, Mitochondrial 1A, EC 2.7.3.2, Mia-CK, Ubiquitous Mitochondrial Creatine Kinase, U-MtCK
Gene Symbol
CKMT1A
Additional Creatine kinase MT 1A Products
Product Documents for Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free
Product Specific Notices for Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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