Creatine Kinase BB Antibody (8E4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59462
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 8E4
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human Creatine Kinase BB (1-381aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Creatine Kinase BB Antibody (8E4) - BSA Free
Western Blot: Creatine Kinase BB Antibody (8E4)BSA Free [NBP2-59462]
Western Blot: Creatine Kinase B Antibody (8E4) [NBP2-59462] - Lane 1: HeLa cell lysates, Lane 2: 293T cell lysates, Lane 2: K562 cell lysates, Lane 2: LnCap cell lysatesImmunocytochemistry/ Immunofluorescence: Creatine Kinase BB Antibody (8E4) - BSA Free [NBP2-59462]
Immunocytochemistry/Immunofluorescence: Creatine Kinase B Antibody (8E4) [NBP2-59462] - Analysis of CKB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Flow Cytometry: Creatine Kinase BB Antibody (8E4) - BSA Free [NBP2-59462]
Flow Cytometry: Creatine Kinase B Antibody (8E4) [NBP2-59462] - Analysis of CKB in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).Applications for Creatine Kinase BB Antibody (8E4) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1063
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Creatine Kinase BB
Alternate Names
B-CK, CKB
Gene Symbol
CKB
Additional Creatine Kinase BB Products
Product Documents for Creatine Kinase BB Antibody (8E4) - BSA Free
Product Specific Notices for Creatine Kinase BB Antibody (8E4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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