Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25417
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2048 + HL1907 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human Collagen I/II/III.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free
Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] -
Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] -
Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] -
Western Blot: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Collagen I/II/III
Alternate Names
Alpha-1 type I collagen, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, OI4, pro-alpha-1 collagen type 1
Gene Symbol
COL1A1
Additional Collagen I/II/III Products
Product Documents for Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free
Product Specific Notices for Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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