CLIC3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89465
![Simple Western: CLIC3 Antibody [NBP1-89465]](https://resources.bio-techne.com/images/products/CLIC3-Antibody-Simple-Western-NBP1-89465-img0010.jpg "Simple Western: CLIC3 Antibody [NBP1-89465]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (92%), Rat (91%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MVLLLKGVPFTLTTVDTRRSPDVLKDFAPGSQLPILLYDSDAKTDTLQIEDFLEETLGPPDFPSLAPRYRESNTAGNDVFHKFSAFIKNPVPAQDEALYQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CLIC3 Antibody - BSA Free (NBP1-89465) is a polyclonal antibody validated for use in WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CLIC3 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: CLIC3 Antibody [NBP1-89465]](https://resources.bio-techne.com/images/products/CLIC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89465-img0011.jpg "Immunocytochemistry/ Immunofluorescence: CLIC3 Antibody [NBP1-89465]")
Immunocytochemistry/ Immunofluorescence: CLIC3 Antibody [NBP1-89465]
Immunocytochemistry/Immunofluorescence: CLIC3 Antibody [NBP1-89465] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies. Antibody staining is shown in green.![Simple Western: CLIC3 Antibody [NBP1-89465]](https://resources.bio-techne.com/images/products/CLIC3-Antibody-Simple-Western-NBP1-89465-img0009.jpg "Simple Western: CLIC3 Antibody [NBP1-89465]")
Simple Western: CLIC3 Antibody [NBP1-89465]
Simple Western: CLIC3 Antibody [NBP1-89465] - Simple Western lane view shows a specific band for CLIC3 in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for CLIC3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Simple Western
1:20
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate(s), separated by Size, antibody dilution of 1:20, apparent MW was 33 kDa.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate(s), separated by Size, antibody dilution of 1:20, apparent MW was 33 kDa.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CLIC3
Alternate Names
chloride intracellular channel 3, chloride intracellular channel protein 3
Gene Symbol
CLIC3
Additional CLIC3 Products
Product Documents for CLIC3 Antibody - BSA Free
Product Specific Notices for CLIC3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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