CHREBP Antibody (2D9NB) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44307
![Immunocytochemistry/ Immunofluorescence: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]](https://resources.bio-techne.com/images/products/CHREBP-Antibody-2D9NB-Immunocytochemistry-Immunofluorescence-NBP2-44307-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Cited:
Western Blot, Chemotaxis
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2D9NB
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant human ChREBP protein between amino acids 600-800.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 31289120). Use in Rat reported in scientific literature (PMID:19363290).
Localization
Cytoplasm, Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for CHREBP Antibody (2D9NB) - BSA Free
Immunocytochemistry/ Immunofluorescence: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]
Immunocytochemistry/Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CHREBP (2D9NB) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]](https://resources.bio-techne.com/images/products/CHREBP-Antibody-2D9NB-Immunohistochemistry-Paraffin-NBP2-44307-img0003.jpg "Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]")
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of human hepatocellular carcinoma using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern in the cytoplasm of cancer cells. Some cells depicted nuclear positivity also while the stroma was largely negative for CHREBP expression.![Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]](https://resources.bio-techne.com/images/products/CHREBP-Antibody-2D9NB-Immunohistochemistry-Paraffin-NBP2-44307-img0002.jpg "Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]")
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of normal human kidney using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern mainly in the cytoplasm (but in some nuclei also) of cuboidal epithelial cells of various ducts and tubules.Applications for CHREBP Antibody (2D9NB) - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation
reported in scientific literature (PMID 31289120)
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1:500 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CHREBP
Alternate Names
BHLHD14, CHREBPcarbohydrate-responsive element-binding protein, Class D basic helix-loop-helix protein 14, MIOWS basic-helix-loop-helix leucine zipper protein, MLX interacting protein-like, MLX interactor, MLX-interacting protein-like, MONDOBWilliams-Beuren syndrome chromosomal region 14 protein, WBSCR14Williams-Beuren syndrome chromosome region 14 protein 1, Williams Beuren syndrome chromosome region 14, Williams-Beuren syndrome chromosome region 14 protein 2, WS-bHLHbHLHd14carbohydrate response element binding protein
Entrez Gene IDs
51085 (Human)
Gene Symbol
MLXIPL
UniProt
Additional CHREBP Products
Product Documents for CHREBP Antibody (2D9NB) - BSA Free
Product Specific Notices for CHREBP Antibody (2D9NB) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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