Cholesterol Oxidase Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-625
Key Product Details
Species Reactivity
Bacteria
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Cholesterol Oxidase [Streptomyces] (Uniprot: P12676)
Reactivity Notes
Reactive with Cholesterol Oxidase from Streptomyces.
Cross reactivity against Cholesterol Oxidase from other sources is unknown
Cross reactivity against Cholesterol Oxidase from other sources is unknown
Specificity
Cholesterol Oxidase
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Cholesterol Oxidase Antibody - BSA Free
![Western Blot: Cholesterol Oxidase Antibody [NB600-625]](https://resources.bio-techne.com/images/products/Cholesterol-Oxidase-Antibody-Western-Blot-NB600-625-img0006.jpg "Western Blot: Cholesterol Oxidase Antibody [NB600-625]")
Western Blot: Cholesterol Oxidase Antibody [NB600-625]
Western Blot: Cholesterol Oxidase Antibody [NB600-625] - Analysis using HRP conjugate of NB600-625. Detection of purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat.![Western Blot: Cholesterol Oxidase Antibody [NB600-625]](https://resources.bio-techne.com/images/products/Cholesterol-Oxidase-Antibody-Western-Blot-NB600-625-img0005.jpg "Western Blot: Cholesterol Oxidase Antibody [NB600-625]")
Western Blot: Cholesterol Oxidase Antibody [NB600-625]
Western Blot: Cholesterol Oxidase Antibody [NB600-625] - Detection of purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat.
Cholesterol Oxidase Antibody
Goat anti Cholesterol oxidase antibody ( lot 6534) was used to detect purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a 1:3000 dilution of primary antibody (ON 4 C in ). Detection shown was using Dylight 488 conjugated Donkey anti goat ( lot 21094 1:10K in TBS/ 1 hr RT). Images were collected using the BioRad VersaDoc System.Applications for Cholesterol Oxidase Antibody - BSA Free
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product has been tested by western blot. This product is suitable to be assayed against 1.0 ug of Cholesterol Oxidase [Microorganism] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Cholesterol Oxidase
Alternate Names
choA, choD, choM, choS, COD
UniProt
Additional Cholesterol Oxidase Products
Product Documents for Cholesterol Oxidase Antibody - BSA Free
Product Specific Notices for Cholesterol Oxidase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...