CDX2 Antibody (9E0K5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57371

Recombinant Monoclonal Antibody
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NBP3-57371-20ul
NBP3-57371-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9E0K5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDX2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive. (Swissprot# Q99626)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Fallopian tube, Pancreas, Lung, Kidney, Placenta, Stomach, Liver, Thyroid, Appendix, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Colon, Brain

Cancer Tissues Tested
Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Thyroid Cancer, Prostate cancer, cervical squamous cell carcinoma, Breast Cancer, Colon Cancer

Positive Controls
Appendix/colon (columnar epithelial cells), Colon Cancer, Pancreas (all ductal and intercalated duct epithelial cells should demonstrate at least weak to moderate, distinct nuclear staining reaction)

Negative Controls
Appendix/colon (leukomonocyte, Fibroblasts), tonsil, liver, Placenta, Breast

Applications for CDX2 Antibody (9E0K5)

Application
Recommended Usage

ELISA

1 ug/mL

Immunohistochemistry-Paraffin

1:3000 - 1:12000

Immunoprecipitation

0.5ug-4ug antibody for 200ug-600ug extracts of whole cells

Western Blot

1:500 - 1:2000
Application Notes
For immunofluorescence on paraffin-embedded tissues, the recommended concentration is 1:100 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, 0.05% BSA, (pH 7.3)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDX2

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Long Name

Caudal-type Homeobox Transcription Factor 2

Alternate Names

CDX3

Gene Symbol

CDX2

Additional CDX2 Products

Product Documents for CDX2 Antibody (9E0K5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CDX2 Antibody (9E0K5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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