CDK2 [p Tyr15] Antibody (SN72-04)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67686

Recombinant Monoclonal Antibody
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NBP2-67686
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN72-04 expressed in HEK293

Concentration

1 mg/ml

View all CDK2 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Tyr15 of human CDK2. (SwissProt: P24941 Human; SwissProt: P97377 Mouse; SwissProt: Q63699 Rat)

Modification

p Tyr15

Localization

Cytoplasm, Cytoskeleton, Endosome, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDK2 [p Tyr15] Antibody (SN72-04)

Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.
CDK2 [p Tyr15] Antibody (SN72-04) Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686]

Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686]

Western blot analysis of Hela cell lysates. Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody (NBP2-67686) at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds

Applications for CDK2 [p Tyr15] Antibody (SN72-04)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:1000
Application Notes
IP: Use at an assay dependent concentration.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for CDK2 [p Tyr15] Antibody (SN72-04)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CDK2 [p Tyr15] Antibody (SN72-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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