CDCP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-860

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NB100-860
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CDCP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PLLNTQEPMEPAE corresponding to C-Terminus according to NP_073753.3.

Specificity

This antibody is expected to recognize isoform 1 (NP_073753.3) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CDCP1 Antibody

Western Blot: CDCP1 Antibody [NB100-860]

Western Blot: CDCP1 Antibody [NB100-860]

Western Blot: CDCP1 Antibody [NB100-860] - (1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer).
Immunohistochemistry-Paraffin: CDCP1 Antibody [NB100-860]

Immunohistochemistry-Paraffin: CDCP1 Antibody [NB100-860]

Immunohistochemistry-Paraffin: CDCP1 Antibody [NB100-860] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CDCP1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
IP successful usage with A549 cells. WB: Approx. 90 kDa band observed in human colon lysates (calculated MW of 92.3 kDa band according to NP_073753.3). An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC successful usage for human endometrium.
IHC: Paraffin embedded Human Prostate.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDCP1

The protein encoded by this gene is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Long Name

CUB Domain Containing Protein 1

Alternate Names

CD318, SIMA135

Entrez Gene IDs

64866 (Human)

Gene Symbol

CDCP1

UniProt

NP_073753. (Human)

Additional CDCP1 Products

Product Documents for CDCP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDCP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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