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CDC42EP3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88382

Novus Biologicals, part of Bio-Techne
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NBP1-88382
NBP1-88382-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLPTIGGSQALMLPLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC42EP3 Antibody - BSA Free

Immunohistochemistry-Paraffin: CDC42EP3 Antibody [NBP1-88382]

Immunohistochemistry-Paraffin: CDC42EP3 Antibody [NBP1-88382]

Immunohistochemistry-Paraffin: CDC42EP3 Antibody [NBP1-88382] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
CDC42EP3 Antibody - BSA Free Western Blot: CDC42EP3 Antibody - BSA Free [NBP1-88382]

Western Blot: CDC42EP3 Antibody - BSA Free [NBP1-88382]

Analysis in control (vector only transfected HEK293T lysate) and CDC42EP3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
CDC42EP3 Antibody - BSA Free

Western Blot: CDC42EP3 Antibody - BSA Free [NBP1-88382] -

CDC42EP3 is upregulated in glioma and correlated with poor prognosis.A Representative photographs of human glioma tissues and normal brain tissues after immunohistochemistry staining of CDC42EP3. The scale bar is 50 um long. B Glioma patients with high expression of CDC42EP3 undergoes relatively shorter overall survival in comparison with those with low CDC42EP3 expression. C The relative mRNA levels of CDC42EP3 in SHG-44 and U251 cells and differences between shCtrl- and shCDC42EP3-harboring cells detected by qPCR and WB. Data were presented by mean with SD (n ≥ 3). **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35365622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CDC42EP3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC42EP3

CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. This protein can interact with CDC42, as well as with the ras homolog gene family, member Q (ARHQ/TC10). Expression of this protein in fibroblasts has been shown to induce pseudopodia formation. [provided by RefSeq]

Alternate Names

Binder of Rho GTPases 2, BORG2CEP3UB1, CDC42 effector protein (Rho GTPase binding) 3, cdc42 effector protein 3, CRIB-containing BORG2 protein, FLJ46903, MSE55-related Cdc42-binding protein, MSE55-related protein

Gene Symbol

CDC42EP3

Additional CDC42EP3 Products

Product Documents for CDC42EP3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CDC42EP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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