CD5 Antibody (CD5/54/F6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34593

Novus Biologicals, part of Bio-Techne
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NBP2-34593-0.1mg
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Key Product Details

Species Reactivity

Human, Rat, Porcine, Bovine, Chicken, Equine, Guinea Pig, Monkey, Rabbit

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CD5/54/F6

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD5 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa450-495) of human CD5 protein (intracellular region). (Uniprot: P06127 )

Localization

Cell surface

Specificity

Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Marker

Mantle Cell Lymphoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32824).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD5 Antibody (CD5/54/F6) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]

Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]

Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593] - Formalin-fixed, paraffin-embedded human tonsil stained with CD5 MAb (CD5/54/F6)
Flow Cytometry: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]

Flow Cytometry: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]

Flow Cytometry: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593] - T cell characterization in canine whole blood cells. From a verified customer review. Image using the Alexa Fluor 700 format of this antibody.

Applications for CD5 Antibody (CD5/54/F6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD5

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Alternate Names

CD5

Gene Symbol

CD5

Additional CD5 Products

Product Documents for CD5 Antibody (CD5/54/F6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD5 Antibody (CD5/54/F6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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