CD47 Antibody (050) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23505
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # 050
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD47 (Uniprot#: Q61735-1; Met1-Lys140).
Clonality
Monoclonal
Host
Rabbit
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
Scientific Data Images for CD47 Antibody (050) - Azide and BSA Free
Western Blot: CD47 Antibody (050) [NBP3-23505] -
Western Blot: CD47 Antibody (050) [NBP3-23505] - NBP3-23505 at 1:500 dilution. Lane A: Mouse brain tissue Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa
Immunoprecipitation: CD47 Antibody (050) [NBP3-23505] -
Immunoprecipitation: CD47 Antibody (050) [NBP3-23505] - CD47 was immunoprecipitated using: Lane A: 0.5 mg Mouse brain Whole Cell Lysate 4 uL NBP3-23505 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-23505, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDaApplications for CD47 Antibody (050) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD47
Alternate Names
CD47, IAP, MER6, OA3
Gene Symbol
CD47
Additional CD47 Products
Product Documents for CD47 Antibody (050) - Azide and BSA Free
Product Specific Notices for CD47 Antibody (050) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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