CD3 epsilon Antibody (145-2C11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30151

Discontinued Product
NBP2-30151 has been discontinued. View all CD3 epsilon products.

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 145-2C11

Concentration

0.5 mg/ml

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

H-2Kb specific cytotoxic T lymphocyte clone BM10-37

Reactivity Notes

Mouse.

Specificity

Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters. Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Applications for CD3 epsilon Antibody (145-2C11)

Application
Recommended Usage

Flow Cytometry

0.5 - 2 ug/2x10^6 cells
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 27246324).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 epsilon

CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (145-2C11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD3 epsilon Antibody (145-2C11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.